Review



α5β1  (Novus Biologicals)


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    Structured Review

    Novus Biologicals α5β1
    FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, <t>α5β1,</t> α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors
    α5β1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/integrin+%CE%B15%CE%B21+m200/Integrin+alpha+5+beta+1+Antibody+(M200+(Volociximab))+-+Chimeric+-+Azide+and+BSA+Free/pm36684084-35-69-71
    Average 93 stars, based on 6 article reviews
    α5β1 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "3D bioprinting of an implantable xeno-free vascularized human skin graft."

    Article Title: 3D bioprinting of an implantable xeno-free vascularized human skin graft.

    Journal: Bioengineering & translational medicine

    doi: 10.1002/btm2.10324

    FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, α5β1, α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors
    Figure Legend Snippet: FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, α5β1, α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors

    Techniques Used: Microscopy, Isolation, Cell Culture, Flow Cytometry, Expressing, Confocal Microscopy, Staining

    Related Articles

    Fluorescence:

    Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin?
    Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); Integrin α5β1 (M200) (NBP2-52680) from Novus Biologicals and purchased through Bio-Techne (Wiesbaden, Germany); Alexa Fluor ® 594 AffiniPure goat anti-mouse IgG (H+L) (115-585-003) from Jackson ImmunoResearch and purchased through Dianova (Hamburg, Germany); goat anti-rabbit IgG-HRP (7074s) and anti-rabbit IgG (H+L) F(ab') 2 Fragment Alexa Fluor ® 594 Conjugate (8889S) from Cell Signaling (Frankfurt am Main, Germany).

    Epifluorescence Microscopy:

    Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin?
    Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); Integrin α5β1 (M200) (NBP2-52680) from Novus Biologicals and purchased through Bio-Techne (Wiesbaden, Germany); Alexa Fluor ® 594 AffiniPure goat anti-mouse IgG (H+L) (115-585-003) from Jackson ImmunoResearch and purchased through Dianova (Hamburg, Germany); goat anti-rabbit IgG-HRP (7074s) and anti-rabbit IgG (H+L) F(ab') 2 Fragment Alexa Fluor ® 594 Conjugate (8889S) from Cell Signaling (Frankfurt am Main, Germany).

    Staining:

    Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin?
    Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); Integrin α5β1 (M200) (NBP2-52680) from Novus Biologicals and purchased through Bio-Techne (Wiesbaden, Germany); Alexa Fluor ® 594 AffiniPure goat anti-mouse IgG (H+L) (115-585-003) from Jackson ImmunoResearch and purchased through Dianova (Hamburg, Germany); goat anti-rabbit IgG-HRP (7074s) and anti-rabbit IgG (H+L) F(ab') 2 Fragment Alexa Fluor ® 594 Conjugate (8889S) from Cell Signaling (Frankfurt am Main, Germany).

    Flow Cytometry:

    Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin?
    Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); Integrin α5β1 (M200) (NBP2-52680) from Novus Biologicals and purchased through Bio-Techne (Wiesbaden, Germany); Alexa Fluor ® 594 AffiniPure goat anti-mouse IgG (H+L) (115-585-003) from Jackson ImmunoResearch and purchased through Dianova (Hamburg, Germany); goat anti-rabbit IgG-HRP (7074s) and anti-rabbit IgG (H+L) F(ab') 2 Fragment Alexa Fluor ® 594 Conjugate (8889S) from Cell Signaling (Frankfurt am Main, Germany).



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    FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, <t>α5β1,</t> α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors
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    FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, <t>α5β1,</t> α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors
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    Image Search Results


    FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, α5β1, α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors

    Journal: Bioengineering & translational medicine

    Article Title: 3D bioprinting of an implantable xeno-free vascularized human skin graft.

    doi: 10.1002/btm2.10324

    Figure Lengend Snippet: FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, α5β1, α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors

    Article Snippet: ECs, FBs, PCs, and KCs cultured under xeno-free conditions were analyzed for surface and intracellular markers expression by flow cytometry and immunofluorescence microscopy using antibodies against: CD31 (WM59; Biolegend), CD45 (2D1; Biolegend), ZO-1 (sc-33725; Santa Cruz), VE-cadherin (sc-6458; Santa Cruz), vWF (ab201336; abcam) claudin-5 (34-1600; Invitrogen), PDGFR-α (16A1; Biolegend), PDGFR-β (18A2; Biolegend), CD90 (5E10; Biolegend), NG2 (9.2.27; Invitrogen), a-SMA (1A4; Invitrogen), FAP (AF3715; Novus Biologics), integrins α2β1 (ab24697; abcam), α5β1 (NBP2-52680; Novus Biologics), αvβ3 (sc-7312; Santa Cruz), α6 (MAB13501; R&D systems), α3 (MAB1345; R&D systems), β4 (NBP1-43369), CK14 (LL002; Novus Biologics), CK10 (EP1607IHCY, abcam), and occludin.

    Techniques: Microscopy, Isolation, Cell Culture, Flow Cytometry, Expressing, Confocal Microscopy, Staining