α5β1 (Novus Biologicals)
Structured Review

α5β1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/integrin+%CE%B15%CE%B21+m200/Integrin+alpha+5+beta+1+Antibody+(M200+(Volociximab))+-+Chimeric+-+Azide+and+BSA+Free/pm36684084-35-69-71
Average 93 stars, based on 6 article reviews
Images
1) Product Images from "3D bioprinting of an implantable xeno-free vascularized human skin graft."
Article Title: 3D bioprinting of an implantable xeno-free vascularized human skin graft.
Journal: Bioengineering & translational medicine
doi: 10.1002/btm2.10324
Figure Legend Snippet: FIGURE 5 Phenotyping characterization of human keratinocytes under xeno-free conditions. (a) Live phasecontrast microscopy images of keratinocytes after isolation from the epidermis of donor foreskin and at the confluency state. (b) The cumulative population doublings of keratinocytes cultured under xeno-free conditions is comparable to KGM-Gold medium. (c) Flow cytometry analysis confirmed the expression of integrins α2β1, α5β1, α6, α3, and β4, but not αvβ3. (d) Confocal microscopy exhibiting CK14, CK10, junctional ZO-1, and intracellular occludin staining. Scale bar = 100 μm. Representative of three independent donors
Techniques Used: Microscopy, Isolation, Cell Culture, Flow Cytometry, Expressing, Confocal Microscopy, Staining
Related Articles
Fluorescence:Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin? Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); Epifluorescence Microscopy:Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin? Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); Staining:Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin? Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); Flow Cytometry:Article Title: S . aureus alpha-toxin monomer binding and heptamer formation in host cell membranes – Do they determine sensitivity of airway epithelial cells toward the toxin? Article Snippet: Antibodies (Ab) were obtained from these sources: Hla-Ab (S7531) from Sigma (Steinheim, Germany); ADAM10 Ecto (MAB1427-100) from R&D Systems and purchased through antikoerper-online.de (Aachen, Germany); Caveolin-1 (7C8) (sc-53564), normal mouse IgG 2b (sc-3879) from Santa Cruz Biotechnology (Heidelberg, Germany); |